I use the Red Sea Iodine Pro test kit. It is easy to use and to interpret the results. Without direct addition of I2 supplement, the test always showed low levels. I have found the addition of 1/2 capful of TLFs Iodine concentrate every week for my 210 system is sufficient. However, I haven't noticed any real change in my coloration, growth, etc. in the six months since starting this protocol. I think you are probably OK w/o supplementation as long as you have a good water change program. You might need supplementation if you have a softy dominated tank.